A New Colorimetric Method for Rapid Detection of Antibiotic Resistance in Escherichia coli Isolates

Background: The quick diagnosis and early initiation of antibiotic therapy in bacteria-induced infections is paramount importance. Accordingly, the rapid identification causative agent, short-term results sensitivity, selection use right antibiotics for treatment further highlights significance this issue. Objectives: This study aimed to develop a new susceptibility testing method provide Escherichia coli clinical isolates report test clinicians short period. Methods: In study, one hundred ten E. were tested. regard, recommended by "Clinical Laboratory Standards Institute (CLSI)" sensitivity isolates, including amoxicillin-clavulanate, cefixime, ceftriaxone, ertapenem, ciprofloxacin, gentamicin, trimethoprim-sulfamethoxazole, nitrofurantoin For quality control, ATCC25922, ATCC35218, Staphylococcus aureus ATCC29213, 13846NTCC strains used. broth microdilution CLSI was used as reference method. Minimum inhibitory concentration values determined, antimicrobial susceptibilities then determined according “European Committee on Antimicrobial Susceptibility Testing (EUCAST)” criteria. next phase, resazurin microplate (RMM) compared. Results: comparison RMM developed present with revealed that calculated essential agreement ratios eight varied from 82.72 100%, categorical ranged 95.45 100%. Conclusions: According findings, highly allows detection quickly (e.g., within 5 hours) such it preferred, especially laboratories limited facilities. However, multi-center studies are routine laboratories.